Foto: JGU
The Thermo Fisher Scientific Talos F200i is a 200 kV high-brightness field-emission (C-FEG) scanning
transmission electron microscope (S/TEM) designed for fast, precise and quantitative characterisation of nanomaterials.
As the centre's new cryo-capable TEM, the Talos F200i is equipped for cryo-electron microscopy with an automatic cryo box, a low-dose exposure technique and a Long Duration dewar providing about 100 hours of liquid-nitrogen supply, complemented by an automatic cryo-cycle to de-ice the cold trap. A phase plate enhances the contrast of weakly scattering, beam-sensitive specimens such as biological and soft-matter samples.
For analytical work the system carries two symmetric Bruker XFlash 6 | 100 EDS detectors for fast elemental analysis from light to heavy elements, and a Segmented Panther STEM system supporting Z-contrast imaging, Differential and Integrated Differential Phase Contrast (DPC/iDPC) and Annular Bright Field (ABF).
For structure determination from nanocrystals the Talos F200i includes a dedicated MicroED package for continuous-rotation 3D electron diffraction, with an optically aligned tilt axis, optimised C2/SA apertures, a beam stop and automated data-acquisition software (EPU-D). Tomographic studies are possible both in real space (TEM and STEM tomography software, compact analytical tomography holder) and in reciprocal space, and 4D-STEM data can be recorded on the Ceta camera by synchronising the STEM probe with the camera readout. This makes the instrument a versatile platform bridging materials science, structural biology and electron crystallography at the EMC-M.
| Acceleration voltage | 80-200 kV |
| Objective lens | C-Twin |
| Gun type | X-CFEG |
| Specified TEM resolution (information limit) | 0.18 nm |
| 1. Camera | Ceta-S 16M (4k x 4k) |
| 2. Camera | TVIPS XF416R |
| Phase contrast | Phase plate |
| Dark field detectors | Segmented Panther STEM: HAADF + on-axis BF/DF (DPC / iDPC / ABF); 4k × 4k STEM acquisition |
| Specified STEM resolution | 0.2 nm |
| Energy dispersive X-ray spectrometer | Dual Bruker XFlash 6 | 100 (2 × 100 mm², 129 eV) |
| Cryo equipment | Automatic cryo box, low-dose exposure, Long Duration dewar (~100 h LN₂) |